Cellular and developmental biology, part b develop mental biology, the second volume on the topic in the methods in cell biology series, looks at methods for analyzing cellular and developmental biology of zebrafish. Zebrafish biology allows ready access to all developmental stages, and the optical clarity of embryos and. The developmental genetics of vertebrate color pattern formation. Frontiers zebrafish as a model for obesity and diabetes. Ccn2a is an injuryinduced matricellular factor that. Aug 20, 2018 zebrafish is a wellestablished model system for developmental biology, human genetics, and human diseases dooley and zon, 2000. Several features have propelled zebrafish to its current prominence in developmental biology and disease modeling.
Special issue zebrafish a model system for developmental. Pdf zebrafish as potential model for developmental. A robust and sensitive zebrafish reporter line for developmental, chemical and neural biology of the circadian clock developmental biology. Regarding neuroscience studies, exm enables the tracing of cellular processes in the zebrafish brain, as well as the imaging of synapses and their biomolecular content and organization. Developmental stages of zebrafish danio rerio embryos and. The importance of zebrafish in biomedical research universidade. Our results on the novel occ phenotype for optical deficiency encourage the use of the occ zebrafish as a model for human visual deficits involving cell differentiation and development. The ability of zebrafish to heal their heart after injury makes them an attractive model for investigating the mechanisms governing the regenerative process.
Xenopus and zebrafish affords newcomers rapid access to a wide variety of key techniques in developmental research, and offers experienced investigators both new techniques from experts who have finetuned them. Frontiers in cell and developmental biology 2020 ghosh m and rihel j. Wholebrain mapping of socially isolated zebrafish reveals that lonely fish are not loners. To achieve this, we use a multitude of model organisms including drosophila, zebrafish, xenopus, and mouse. The zebrafish danio rerio has become an important model organism for developmental biology and bio medical research in general, and it is the model. Introducing undergraduates to developmental biology and genetics in a large introductory laboratory class allison dcosta and iain t. Special issue zebrafisha model system for developmental. Pdf we have taken advantage of the strengths of the zebrafish model system to introduce developmental biology and genetics to. Downloadable pdf reed and jennings 2011 on the care, handling, and. We describe a series of stages for development of the embryo of the zebrafish, danio.
Zebrafish as a model for developmental biology and. Zebrafish as a model for developmental biology and toxicology. Zebrafish are vertebrates often used for in vivo studies, because they are relatively small in size and the embryonic and larval development is ex utero and fast, since within 5 days postfertilization dpf, all major organ systems are formed kimmel et al. Liver is one of the largest internal organs in the body and its importance for metabolism, detoxification and homeostasis has been well established. A paradigm for development and evolution of adult patterns. Female zebrafish spawn every 23 days and produce several hundred eggs in each clutch. It is due to talent of chris small and his production staff. Epiboly in zebrafish emrbyos developmental biology interactive. Zebrafish development and genetics course marine bio logical laboratory. Highlights on selected microscopy techniques to study. Singlecell resolution is challenging due to different issues such as sample size, the scattering of. The zebrafish, danio rerio, is tropical freshwater fish and a very popular model organism for scientific research in the fields of development, vertebrate processes, genetics, and more. Studies reported briefly here demonstrate that one can use gene expression in the zebrafish embryo to find new components of.
For example, cell behavior during zebrafish embryo cleavage stages was successfully reconstructed in a threedimensional 3d imaging by combining shg with. May 09, 2018 among the model organisms, the zebrafish danio rerio is one of the best leading models to study developmental biology, cancer, toxicology, drug discovery, and molecular genetics. We show that, at this stage, both pancreas and liver progenitors are symmetrically localized in two broad domains relative to the dorsal organizer. Abstract we describe a series of stages for development of the embryo of the zebrafish, danio brachydanio rerio. Zebrafish development developmental biology microbe notes. Zebrafish resources on the internet ilar journal oxford academic. Using zebrafish in a developmental biology lab course to. The growing interest in zebrafish research was paralleled by an increase in tools and methods. Ccn2a is an injuryinduced matricellular factor that promotes. Download pdf human embryology developmental biology ebook. Apr 22, 2020 this effective technique provided a glance at the dynamics of cytological construction during embryogenesis, which added valuable findings to the research of developmental biology 24, 25.
Jan 21, 2021 zebrafish as a model organism for developmental disorders. In the field of developmental biology, zebrafish has been critical in identifying the components of many signalling pathways, the mechanisms behind gastrulation movements and neuronal migration, and the genetic and morphogenetic basis of the development of organs such as the heart, brain, liver, and skeleton. Molecular methods in developmental biology xenopus and. William detrich iii, monte westerfield, and leonard i. Written in a clear and accessible style, the third edition of this popular textbook has been expanded and updated in addition, an accompanying website provides instructional materials for both student and lecturer use, including animated. Specification of zebrafish pgcs by inheritance of germ plasm. We mainly focused on topics related to the specification of hepatoblasts from endoderm, the. We have taken advantage of the strengths of the zebrafish model system to introduce developmental biology and genetics to undergraduates in their second semester of the introductory biology course at emory. Zebrafish have been used predominantly in developmental biology. The zebrafish is an omnivorous vertebrates and consumes zooplankton, insects, insect larvae and phytoplankton.
Essential developmental biology is a comprehensive, richly illustrated introduction to all aspects of developmental biology. A combination of methodologies from functional morphology and developmental biology has allowed us to characterize feeding mechanics of firstfeeding larval zebrafish danio rerio. Dear colleagues, the birth of zebrafish danio rerio research is, for many of us, linked to the historic publication of george streisinger and colleagues nature 1981. We designed a 6week laboratory module based on research being undertaken by faculty in the department, and incorporated experiments that used current research methods including. Also, the zebrafish development is quite fast, with precursors to all major organs developing within 36 postfertilization. Zebrafish eggs are relatively transparenta characteristic that makes it a very desirable model organism for development biology study. In this study, we show that the gene cellular communication network factor 2a ccn2a, previously known as ctgfa, is induced in endocardial cells in the injured tissue and regulates cm proliferation and repopulation of the damaged tissue. From embryonic pattern to form volume 6 in the current topics in developmental biology series highlights new advances in the field, with this new volume presenting interesting chapters on d. Chapters cover such topics as cell biology and developmental and neural biology.
The zebrafish transcriptome during early development the three earliest developmental stages were similar when comparing highly expressed genes, whereas the 50% epiboly stage differed from the other three stages in the identity of highly expressed genes, number of uniquely expressed genes and enrichment of go molecular functions. Targeted cell ablation in zebrafish using optogenetic. Culled from previously published chapters in methods in cell biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of the cellular and developmental biology of zebrafish. Xenopus and zebrafish affords newcomers rapid access to a wide variety of key techniques in developmental research, and offers experienced investigators both new techniques from experts who have finetuned them for best results, and a plethora of timesaving tips. The study of zebrafish as a leading model organism for developmental biology is rapidly expanding. Mione mc, blader p, del bene f, trompouki e and bianco ih.
Further study in zebrafish pigment cells is essential, since they provide many opportunities to learn the nature of the human skin color. Functional morphology and developmental biology of zebrafish. The developmental genetics of vertebrate colour pattern formation. In zebrafish, mutations per gene can be obtained per. Current topics developmental biology pdf nussleinvolhard, c.
The deep cells of the blastoderm rearrange to form the hypoblast and the epiblast, which form the embryonic anlagen. Contrary to kinematic patterns commonly seen in adult teleosts, larval zebrafish showed no lateral abduction during the expansive phase of a suctionfeeding event. Realtime in vivo monitoring of circadian ebox enhancer activity. Sexspecific perceptual space for a vertebrate basal social aggregative behavior.
Threedimensional live embryo imaging of the zebrafish eye is excellent for research on developmental diseases involving visual function. Dopaminergic and noradrenergic circuit development in. The remainder of this chapter will focus on the advantages and applications of zebrafish in developmental biology and toxicology research. The zebrafish danio rerio is one of the most important vertebrate model organism in developmental biology grunwald and eisen, 2002. Developmental stages of zebrafish danio rerio embryos. In addition, they develop rapidly, so that at 24 hours after fertilization, the embryo has formed most of its tissues and organ primordia and displays the. Pdf functional morphology and developmental biology of. Functional morphology and developmental biology of. Zebrafish danio rerio as a model organism intechopen. Although the zebrafish model provides an important platform for the study of developmental biology, recent work with the zebrafish model has extended its application to a wide variety of experimental studies relevant to human disease. They can be raised so that mutants can be readily screened and propagated. Zebrafish are a prominent system for obtaining a basic research understanding of developmental biological processes, and the use of zebrafish as an animal model for human disease continues to grow. Altogether the tools available and the plasticity of this biological model allowed.
Developmental plasticity in the thermal tolerance of. In the present study, foldscope is used as an innovative tool to study the developmental stages and toxicological analysis of the zebrafish embryos. Pdf essential developmental biology download ebook the home. The zebrafish model has recently emerged as a significant experimental system for developmental biology studies. Calcineurin controls proximodistal blastema polarity in. The teleost retina as a model for developmental and. Pdf highlights on selected microscopy techniques to study. Blocking explant morphogenesis results in failure to correctly pattern the neural tissue.
Zebrafish as a developmental model organism for pediatric. It was followed by a series of seminal papers on zebrafish developmental staging and cell lineage studies by charles kimmel and. Developmental biology of zebrafish dawid 2004 annals. Overall, zebrafish provide a costeffective, wellestablished vertebrate development model amenable to highthroughput screening. Stages of embryonic development of the zebrafish june 2015. Origin of the zebrafish endocrine and exocrine pancreas. In addition, the zebrafish is increasingly used as a genetic model organism for aquaculture species and in toxicogenomics and also to generate zebrafish disease. Studies reported briefly here demonstrate that one can use gene expression in the zebrafish emb. In this chapter, the vertebrate model organism, zebrafish danio rerio, will be discussed in the context of its amenability to developmental biology and toxicology.
Here, we report a detailed fate map of the zebrafish pancreas at the early gastrula stage of development 6 hours postfertilization. Feb 04, 2021 zebrafish have transparent embryos that develop outside the mother which is an important feature for microscopy. This work detailed the development of zebrafish genetic procedures and clonal lines. This gene, slc24a5, is shared by both humans and zebrafish and makes melanosomes less abundant, less concentrated and smaller in lighterskinned humans or lightstriped zebrafish. Developmental biology of zebrafish dawid 2004 annals of. Sunday evening status of the zebrafish as a model for vertebrate genetics and development some features of the zebrafish and its embryo make it an attractive system for learning about development and how genes control development. Introduction to the zebrafish marine biological laboratory. To evaluate developmental plasticity in thermal tolerance of zebrafish danio rerio, common. Pdf highlights on selected microscopy techniques to.
The phylogenetic position of the zebrafish danio rerio. Contrary to kinematic patterns commonly seen in adult teleosts, larval zebrafish showed no lateral abduction during the expansive phase of a. The zebrafish, danio brachydanio rerio, has become one of the most widely studied model systems in developmental biology. The department of cell, developmental and regenerative biology at the icahn school of medicine at mount sinai explores the development, patterning, and regeneration of cells and organs. At early stages of development, vasa rna and germ plasm can also be detected outside of the main four clusters small blue dots, but this material is progressively degraded. In mammals, perivascular cells impede axon regeneration after spinal cord injury through secretion of an inhibitory ecm. At the 4cell stage, the germ plasm containing vasa mrna is enriched in the vicinity of the cleavage furrows blue stripes. The articles in this special issue underscore the diversity and utility of zebrafish research for basic and biomedical developmental biology. Jan 01, 2018 overall, zebrafish provide a costeffective, wellestablished vertebrate development model amenable to highthroughput screening. Pdf essential developmental biology download ebook the. Normal table of postembryonic zebrafish development. We designed a 6week laboratory module based on research being undertaken by faculty in the department, and incorporated experiments that used current research methods including bioinformatics. Zebrafish have been used in course modules to demonstrate second messenger systems 32, oxygen consumption 33, and nervous system development.
We present a dnabased phylogeny of zebrafish and other species of the genus danio, and the genera rasbora, puntius and cyprinus. The methods in this volume were handselected by the. Collegiate classrooms are also recognizing zebrafish as a model organism for guided inquiry, with several publications in the past decade using zebrafish as a tool to teach developmental biology. We define seven broad peri ods of embryogenesisthe zygote, cleavage, blas tula, gastrula, segmentation, pharyngula, and hatching periods. The biology and genetics of zebrafish has become a very popular system in developmental biology especially because of their suitability for genetics and the transparent embryo of this small tropical fish. Expansion microscopy of zebrafish for neuroscience and.
628 1290 1789 71 1313 437 1229 1635 1240 780 1112 294 1730 455 1601 1013 280 238 860 680 542 1277 1873 862 393 1732 527 366 1678 1276 121 393 643 1654 877 1140 1271 384 605