Reserpine is well known bioactive compound isolated from rauvolfia serpentina. Delftia tsuruhatensis wgruombt1, a novel rhizobacterium. It is a compound commonly used in asian medicine, including traditional ayurveda medicine native to india. The discovery of genus rauwolfia dates back to the 16th century and around species are known till now among which the most useful variant available in india of commercial importance is r. Biological activity of endophytic fungi of rauwolfia serpentina benth. Identification of suitable substitute for sarpagandha. Spiridonova e v, d m adnof, i o andreev and va kunakh. Quantification of reserpine content and antibacterial activity of rauvolfia serpentina l. Antimicrobial effects of indian medicinal plants against acneinducing bacteria gs kumar1, kn jayaveera2, ck ashok kumar3, umachigi p sanjay4, bm vrushabendra swamy5, dv kishore kumar6.
Us2870140a therapeutic agents derived from rauwolfia. A clinical trial of rauwolfia serpentina in essential hypertension by rustom jal vakil fromthe cardiological department, kingedwardmemorialhospital, bombay,india received january 4, 1949 inacasualperusal ofpapersonhypertension, one comes across well over a hundred socalled hypotensive remedies alleged to possess the property of. It calms the central nervous system, regulates heart rate and reduces high blood pressure. Aug 10, 2015 the bacterial strain designated as wgruombt1 isolated from rhizosphere of rauwolfia serpentina exhibited broad. Hence these two plant products inhibited ace activity very significantly. The present study is aimed at finding out an appropriate substitute for the endangered species r. Rauwolfia serpentina have antioxidant 5, 8 and antimicrobial activities 89, which were reported in earlier studies.
A clinical trial of rauwolfia serpentina in essential hypertension by rustom jal vakil fromthe cardiological department, kingedwardmemorialhospital, bombay,india received january 4, 1949 inacasualperusal ofpapersonhypertension, one comes across well over a hundred socalled hypo tensive remedies alleged to possess the property of loweringthebloodpressure. From the dried roots of rauwolfia serpentina were isolated five new indole alkaloids, nbmethylajmaline 1, nbmethylisoajmaline 2, 3hydroxysarpagine 3, yohimbinic acid 4, isorauhimbinic acid 5, a new iridoid glucoside, 7epiloganin 6, and a new sucrose derivative, 6o3,4,5trimethoxybenzoylglomeratose a 7, together with 20 known compounds. Phytochemical and antimicrobial study of rauwolfia serpentina. Pathogenicity and antimicrobial activity of seedbefore fusarium. Jul 16, 2019 reserpine 6 is an indole alkaloid that had been procured from rauwolfia serpentina and well known natural compound with potent epi activity.
Antibacterial activity, rauvolfia tetraphylla, human pathogens, disc diffusion, dry fruit extracts, growth inhibition. In a study, antibacterial activity of the methanol extracts of leaf, root of rauvolfia serpentina was tested against grampositive and gramnegative bacteria. Walde the root of sarpgandha is a species of flowering plant in the family apocynaceae has been traditionally used in ayurveda for many years to treat the variety of diseases of that at been traditionally apper to bear little similar to. The mtt assay was used to analyze the ntiproliferative a activity of leaf and root of extracts rauwolfia serpentina on human cervical. The principle alkaloid of rauwolfia serpentina is reserpine 10. In vitro antimicrobial activity of spices and medicinal. Oct, 2020 background sarpagandha is an important ayurveda drug used for treating many diseases including high blood pressure.
We aimed to find the in vitro antibacterial activity of ethanolic extracts of 16 different traditionally used medicinal plants of nepal against clinical and 2 reference bacterial. The hereby research work was carried out to investigate the antimicrobial activity of solvents as well as aqueous extracts of rauwolfia serpentina roots. Rauwolfia serpentina belongs to family apocynaceae. Antibacterial activity and preliminary phytochemical.
Bacterial strain bacteria plants zone of inhibition mm gram positive staphylococcus aureus azadiracta indica. Considerable research works have been done not only on the pharmacological and phytochemical aspects, but also on the antimicrobial properties of higher plants. Therefore, it is necessary to find alternative antimicrobial drugs and the present trend is focused on medicinal plants. Evaluating the therapeutic efficiency and drug targeting. Pdf therapeutic uses of rauwolfia serpentina pubs gupta. Its root is ground into a powder and packaged in this form or sold in tablets or capsules. The antibacterial activity of the methanol extracts was evaluated by determination of minimum inhibitory. The drug has the distinct advantage among hypotensive agents in being associated with no serious untoward sideeffects. The staphylococcus aureus was the most resistant bacteria while pseudomonas mendocina was the most susceptible bacteria. The antibacterial activity of crude extract is shown in table 1. Screening of indian medicinal plants and their potentials.
The plant was screened for the endophytic fungi and their associated biological activities to assess if these fungi have any significant hypocholesterolemic, antioxidant and antimicrobial activities. Pdf methanolic root extract of rauwolfia serpentina. The extracts showed maximum activity against staphylococcus aureus, streptococcus pyogens, klebsiella pnemonia and pseudomonas aurogonosa. Antibacterial activity of individual plant bark extracts. Phytochemical screening and antimicrobial activity of leaf. Antimicrobial strains showed prevalence of polyketide synthase pks typeii 85% followed by pks typei 14% encoded in the genomes. The bacterial strain designated as wgruombt1 isolated from rhizosphere of rauwolfia serpentina exhibited broadspectrum antifungal activity and also improved early. It calms the central nervous system, regulates heart. Antimicrobial biosynthetic potential and genetic diversity. In vivo antimalarial activity and toxicological effects of. Jul 01, 2016 study of the antibacterial activity of the agnp the antibacterial activity of the biosynthesized agnps from r. The aim of this study was evaluate the antimicrobial activity ofsome tannins and polyphenolic compounds extracts leaves of origanum vulgare collected from the mountainous region of boumerdes against a diverse range of microorganisms comprising bacteria and yeasts.
Quantification of reserpine content and antibacterial activity of. Rauwolfia serpentina sarpagandha is a glabrous perennial undershrub. However, its antidiabetic potential has been currently described in both alloxantreated and normoglycemic mice. Study of the antibacterial activity of the agnp the antibacterial activity of the biosynthesized agnps from r. In the present investigation, comparison of antimicrobial activities of different spices, curcuma longa, zingiber officinale, and mentha arvensis, and medicinal herbs, such as withania somnifera, rauvolfia serpentina, emblica officinalis, terminalia arjuna, and centella asiatica, was evaluated. Amongst the plant species screened, methanol extract of rauvolfia serpentina root showed best. Present work aimed to investigate the in silico activity of the alkaloids of roots of rauwolfia serpentina as inhibitors of 3hydroxy3methylglutarylcoa reductase hmgcr. Rauvolfia serpentina an overview sciencedirect topics. For this purpose, the threedimensional 3d structure of the protein hmgcr pdb id. Antimicrobial activity of fungal extract were tested against four bacteria namely, escherichia coli mtcc730, enteroccocus faecalis mtcc2729, salmonella enterica ser. Rauwolfia serpentina is reported in an ayurvedic medicinal system for centuries, for the treatment of various ailments such as snakebites, insomnia, hypertension, and insanity. In lung, inhibitory potency of allium sativum was found to be inhibitory units with 60% inhibition and rauwolfia serpentina was 17 inhibitory units with 57% inhibition table 2.
J jawaharlal nehru technological university college of engineering, ananthpur 01, andhra pradesh, india. Rauwolfia serpentina also spelled rauvolfia serpentina, a member of the apocynaceae family and known as indian snake root or sarpagandha, is one of the most important medicinal plants for treating high blood pressure. Antiarrhythmic activity of the total alkaloids from a. Use of rauwolfia serpentina for antihypertensive activity deepali namdeo tapre, dr. The detailed spectrum of rauwolfia serpentina in the management of diabetic dyslipidemia has not been reported yet.
Biological activity of endophytic fungi of rauwolfia. In vitro antimicrobial activity of spices and medicinal herbs. A wide range of bacterial species, including staphylococcus spp. Jul 24, 2020 rauwolfia serpentina is used as drug since ancient times. In nigerian, the plant is commonly referred to as asofeyeje yoruba, akanta igbo and aada hausa 2. Whereas, achyranthes aspera showed a minium zone of mm against bacillus subtilis similar to the effect of amikacin antibiotic. Recent work revealed the potential of several herbs as sources of drugs iwu, 2002. Antimicrobial and antioxidant activity of crude extracts. Kapalka, in nutritional and herbal therapies for children and adolescents, 2010 rauwolfia. There were still about 121 chemical substances identified from different medicinal plant which are used throughout the world. Rauwolfia rauwolfia serpentina, also spelled ravolphia, is a medicinal shrub in the milkweed family. The screening of plant extracts and plant products for antimicrobial activity has.
Antiarrhythmic activity of the total alkaloids from arauwolfia serpentina tissue culture. Therefore the present study was designed to identify the phytochemical profile of mret of r. Request pdf phytochemical and antimicrobial study of rauwolfia serpentina the ethanolic extract of powder root of rauwolfia serpentina was screened in regards to the effects of the extracted. Antimicrobial biosynthetic potential and genetic diversity of. The disc diffusion method confirmed the antimicrobial activity of rauwolfia serpentina at concentrations ranging from50. Phytochemical screening and antimicrobial activity of. Antiarrhythmic activity of the total alkaloids from arauwolfia serpentina. Endophytic fungi colletotrichum gloeosporioides, penicillium sp. Nigerian journal of pharmaceutical research 122016.
Pdf invitro antimicrobial activity of roots of rauwolfia serpentina. Issr marker based dna profiling studies in rauwolfia species. Initially genomic dna was isolated from the fresh leaves 1 gram of 24 months old rauwolfia tetraphylla, r. Antihypertensive and antihyperlipidemic activity of. Dynamics of genome changes in rauwolfia serpentina callus tissue upon the switch to conditions of submerged cultivation. Rauwolfia serpentina ameliorates hyperglycemic, haematinic. Pdf the main objective of present study was to study the antibacterial effect of rauwolfia serpentina on staphylococcus aureus. About 80 alkaloids are isolated from rauvolfia species among them reserpine is most important principal active constituent. Antibacterial activity and preliminary phytochemical screening of.
In vitro antimicrobial activity screening of bacteria. The endophytes were characterized as colletotrichum gloeosporioides. Archana nath northeastern hill university, hill univ. Rauwolfia serpentina for high blood pressure, adhatoda zeylanica for bronchitis, tylophora indica for asthma and whooping cough. Twenty fungal isolates were recovered from different parts of the host plant and they were characterized for their morphological features through. The plant is known to possess antidiarrheal, antimicrobial activity apart from using it for the treatment of circulatory disorders, rheumatism, hypertension. The worldwide increase of multidrug resistance in both community and healthcare associated bacterial infections has impaired the current antimicrobial therapy, warranting the search for other alternatives. Antimicrobial activity is studied by well diffusion method using four various.
In vitro antimicrobial activity screening of bacteria endophytic to ethnomedicinal plant rauvolfia serpentina l. The works done concern only the antimicrobial activity. It is given in many disorders like worm infestation, insomnia, epilepsy, fever, obesity, mental ailments, influence of evil spirit etc. Tsarenko, quantitative determination of the majorrauwolfia alkaloids by physicochemical methods. Indole alkaloids and other constituents of rauwolfia. Shannons diversity index showed that azadirachta indica 1. Different extraction solvents acetone, methanol, ethanol, and water were used and. Search for new antibacterial agents should be continued by screening many plant families. An investigation on the antibacterial activity of rauvolfia. Kumari s, shukla g, rao as, the present status of medicinal plants aspects and prospects, international journal of research in pharmaceutical.
Issr marker based dna profiling studies in rauwolfia. The plant rauwolfia serpentina is a major source of biologically active indole alkaloids. Rauwolfia serpentina is wellreported in traditional medicines for the treatment of hypertensive and neurological disorders. Antimicrobial and antioxidant activity of crude extracts of rauvolfia caffra var. Apr 27, 20 rauwolfia serpentina benth is an ethnomedicinal plant used as antihypertensive remedy by the ethnic tribes of northeast india. Authors abstract of dissertation for the degree of candidate of pharmaceutical sciences in russian, kharkov 1969. Acalphya indica showed no activity against any of the three bacteria used. Antimicrobial strains showed prevalence of polyketide synthase pks typeii 85% followed by. Issn in vitro antibacterial activity of certain folk. It is reported that the plant also has antimicrobial activity. Aqueous extracts of rauwolfia serpentina leaves and allium sativum cloves were used in the enzyme assay to determine their effect on kidney and lung ace. Antimicrobial and antioxidant activity of crude extracts of. Some recent reports have shown good antimicrobial activity of the extracts of leaves and roots of r.
Evaluation of antibacterial activity of some traditionally. In general, the fungal extracts inhibited the growth of test organisms except e. Isolated endophytes were screened for their antibacterial activity against pathogenic bacteria. Quantification of reserpine content and antibacterial. Research article in vitro antimicrobial activity of. Endophytic fungi isolated from rauvolfia serpentina, a well known indian medicinal plant, is used in ayurveda for treatment of many diseases. Antimicrobial and phytochemical analysis of bark of some. Antimicrobial effects of indian medicinal plants against.
These data revealed that leaf extracts of heliotropium indicum exhibited significant antimicrobial activity. Us369352a therapeutic agents derived from rauwolfia serpentina us2870140a en. The latest addition to the list of pharmacological agents that are effective in lowering the blood pressure is rauwolfia serpentina and its various extracts. The content of genomic dna isolated from 1 gram of fresh leaves of these five species. Rauwolfia rauvolfia plant belongs to the family apocynaceae and occurs in terrestrial habitats of tropical and subtropical regions with over 50 species that are distributed worldwide. Rauvolfia serpentina phytochemical pharmacological and. Mentha arvensis, and medicinal herbs, such as withania somnifera, rauvolfia serpentina, emblica officinalis, terminalia arjuna, and. About drug sarpagandhasarpagandha rauvolfia serpentina is a species of flowering plant in the family apocynaceae. The aim of this study was to quantify reserpine content and evaluate the antibacterial activity of methanol extracts of r. Antimicrobial effects of indian medicinal plants against acne. In this present study, a protocol was developed for the dna profiling of medicinally important rauwolfia species 05 species by issrpcr method. Oct 24, 2017 the disc diffusion method confirmed the antimicrobial activity of rauwolfia serpentina at concentrations ranging from50.
Based on morphological, biochemical and 16s rrna gene sequence analyses, the strain bt1 was identified as delftia tsuruhatensis kf727978. Rauvolfia serpentina is the genuine source plant for sarpagandha and it is a critically endangered species belonging to the family apocynaceae. In vitro antimicrobial activity screening of bacteria endophytic to. The plant possess tuberous root with pale brown cork and elliptic to lanceolate or obovate leaves in whorls of three. Rauwolfia serpentina ameliorates hyperglycemic, haematinic and antioxidant status in alloxan induced diabetic mice muhammad bilal azmi1, 2 and shamim a. The results of linearity of ace activity in kidney and lung was established with hhl as substrate for the incubation period of 30 min at 37c. Biological application of green silver nanoparticle. In vitro callus induction and antioxidant activity of rauwolfia vomitoria afzel.
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